Phytosphingosine promotes megakaryocytic differentiation of myeloid leukemia cells

BMB Reports

Ikjoo Seong^1¹  Yerim Her^1²  Sang Hee Han^1³  Sera Park^2⁴  Jusong Kim^1⁵ 

[1] BK21 Plus R-FIND Team, College of Pharmacy, Dongguk University, Goyang 10326^4;Bio-informatics Science, Ewha Womans University, Seoul 03760^3;Departments of^1;Doosan Corporation, Suwon 16229, Korea^5;Life Science^2

关键词: K562;   

DOI  :  

学科分类：生物化学/生物物理

来源: Korean Society for Biochemistry and Molecular Biology

【 摘 要 】

We report that phytosphingosine, a sphingolipid found in many organisms and implicated in cellular signaling, promotes megakaryocytic differentiation of myeloid leukemia cells. Specifically, phytosphingosine induced several hallmark changes associated with megakaryopoiesis from K562 and HEL cells including cell cycle arrest, cell size increase and polyploidization. We also confirmed that cell type specific markers of megakaryocytes, CD41a and CD42b are induced by phytosphingosine. Phospholipids with highly similar structures were unable to induce similar changes, indicating that the activity of phytosphingosine is highly specific. Although phytosphingosine is known to activate p38 mitogen-activated protein kinase (MAPK)-mediated apoptosis, the signaling mechanisms involved in megakaryopoiesis appear to be distinct. In sum, we present another model for dissecting molecular details of megakaryocytic differentiation which in large part remains obscure.

【 授权许可】

Unknown