Green synthesis of silver nanoparticles and biopolymer nanocomposites: a comparative study on physico-chemical, antimicrobial and anticancer activity

Bulletin of Materials Science

RAMASUBBA REDDY PALEM^1¹  SHIMOGA D GANESH^1²  ZUZANA KRONEKOVA^2³ 

[1] Centre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Tˇr. T. Bati 5678, 760 01 Zlin, Czech Republic^1;Department for Biomaterials Research, Polymer Institute, Slovak Academy of Sciences, Dubravska cesta 9, 845 41 Bratislava, Slovakia^2;Institute of Virology, Biomedical Centre, Slovak Academy of Sciences, Dubravska cesta 9, 845 05 Bratislava, Slovakia^3

关键词: Biogenic silver nanoparticles;    biopolymer nanocomposites;    nanoparticles stability;    antimicrobial activity;    anticancer activity.;   

DOI  :  

学科分类：材料工程

来源: Indian Academy of Sciences

【 摘 要 】

The current report was intended towards comparative study of green-synthesized biogenic Rhubarb silver nanoparticles (RS-AgNPs) and chitosan crosslinked silver nanocomposites (CSHD-AgNCs). The physico-chemical characterization was done by UV–visible, FTIR, scanning electron microscopy (SEM), transmission electron microscopy (TEM), EDX, TGA, XRD and zeta potential ($\zeta$). The analysis and spectroscopic characterization was done by SEM and TEM and their results reveal that the nanoparticles are spherical in shape, with average size ranges from 5 to 50 nm, and was gatheredby face centered cubic (FCC) structure throughout the polymer matrix and stable without any protecting or capping reagents over 450 days. The antimicrobial property of RS-AgNPs and CSHD-AgNCs ($\zeta = +29.6$ and $+$32.8 mV) was evaluated against E. coli and S. aureus and showed an effective inhibitory property. The RS-AgNPs and CSHD-AgNCs were assessedfor their anticancer activity against HeLa cell line by MTT method, and it reveals a dose–response activity, time and cell line-dependent cytotoxicity. Based on the results obtained, the RS-AgNPs exhibited higher toxicity over CSHD-AgNCsafter 24 h incubation of HeLa cells with different concentrations and is negligible for the aqueous Rhubarb extract. It was concluded that the changes in anticancer activity towards HeLa cells due to biological activity of silver nanoparticles depend on their method of biosynthesis and their physico-chemical nature.

【 授权许可】

CC BY