期刊论文详细信息
The Journal of Veterinary Medical Science
mRNA expression of tumor-associated genes in canine grade I meningiomas
Ken OKABAYASHI1 
[1] Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan
关键词: canine meningioma;    housekeeping genes;    real-time RT-PCR;    tumor-associated genes;   
DOI  :  10.1292/jvms.18-0491
学科分类:兽医学
来源: Japanese Society of Veterinary Science
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【 摘 要 】

This study was undertaken to establish a method for measuring mRNA expression by using real-time RT-PCR in the diagnosis of canine meningiomas. When performing real-time RT-PCR, it is essential to include appropriate control tissues and to select appropriate housekeeping genes as an internal standard. Based on the results of our study, RPS18 constitutes a suitable internal standard for the comparison of mRNA expression between normal meninges and meningiomas. The results showed increased mRNA expression of VEGFA and EGFR; however, mRNA expression of KDR was reduced. Measuring mRNA expression by using real-time RT-PCR with appropriate control tissues and internal standards can provide useful information to understanding the pathogenesis of canine meningiomas, which corresponds with immunohistochemical findings.

【 授权许可】

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