mRNA expression of tumor-associated genes in canine grade I meningiomas

The Journal of Veterinary Medical Science

Ken OKABAYASHI¹ 

[1] Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan

关键词: canine meningioma;    housekeeping genes;    real-time RT-PCR;    tumor-associated genes;   

DOI  :  10.1292/jvms.18-0491

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

This study was undertaken to establish a method for measuring mRNA expression by using real-time RT-PCR in the diagnosis of canine meningiomas. When performing real-time RT-PCR, it is essential to include appropriate control tissues and to select appropriate housekeeping genes as an internal standard. Based on the results of our study, RPS18 constitutes a suitable internal standard for the comparison of mRNA expression between normal meninges and meningiomas. The results showed increased mRNA expression of VEGFA and EGFR; however, mRNA expression of KDR was reduced. Measuring mRNA expression by using real-time RT-PCR with appropriate control tissues and internal standards can provide useful information to understanding the pathogenesis of canine meningiomas, which corresponds with immunohistochemical findings.

【 授权许可】

Unknown