3D-Printed, Pocket-Size Diffusion Cells for Skin Permeation Investigation

Proceedings

Athanassiou, Athanassia¹  Bertorelli, Rosalia²  Spanò, Raffaele³  Diaspro, Alberto⁴  Suarato, Giulia⁵  Surdo, Salvatore⁶ 

[1] Author to whom correspondence should be addressed.;D3 PharmaChemistry, Istituto Italiano di Tecnologia, 16163 Genova, Italy;In vivo Pharmacology Facility, Istituto Italiano di Tecnologia, 16163 Genova, Italy;Nanophysics, Istituto Italiano di Tecnologia, 16163 Genova, Italy;Smart Materials, Istituto Italiano di Tecnologia, 16163 Genova, Italy;These authors equally contributed to the work.

关键词: transdermal drug-delivery;    molecule diffusivity;    bottom-up approach;    lab-on-chip;   

DOI  :  10.3390/proceedings2130945

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

Here we present a novel, compact 3D-printed diffusion cell as an in vitro tool for skin permeation investigation. As proof-of-concept, a diffusion cell for studying the permeation of a model molecule (FITC-dextran, 4 kDa) through explanted mice skin is fabricated and characterized. Good viability of the tissue up to 24 h incubation in the cell is demonstrated via MTT assays. The real-time diffusion of the molecule by means of fluorescence microscopy allowed the determination of its diffusivity through the skin (~2·10−10 m2/s). Our results open the door for the real-time, high-throughput and cost-effective investigation of skin in any labs.

【 授权许可】

CC BY