Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae)

Biological research: BR

Karla Quiroz¹  Pablo Cceres²  Basilio Carrasco³  Jos Pico-Mendoza⁴  Rolando Garcia-Gonzales⁵ 

[1] Centro de Biotecnologa de los Recursos Naturales, Departamento de Ciencias Forestales, Universidad Catlica del Maule, Talca, Chile;Facultad de Agronoma, Pontificia Universidad Catlica de Chile, Santiago de Chile, Chile;Facultad de Ciencias Agrarias, Universidad de Talca, Talca, Chile;Facultad de Ingeniera Agronmica, Universidad Tcnica de Manab, Portoviejo, Ecuador;Sociedad de Investigacin y Servicios, BioTECNOS Ltda, Talca, Chile

关键词: Microsatellite;    Pyrosequencing;    Chilean berry;    Gaultheria pumila;   

DOI  :  10.1186/s40659-018-0193-4

学科分类：生物科学（综合）

来源: BioMed Central

【 摘 要 】

Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is a very important Ericaceae endemic to Chile with a large commercial potential. Its resistance to different abiotic conditions makes it a valuable target for genetic improvement. Ten polymorphic simple sequence repeat (SSR) loci were isolated from Gaultheria pumila using new-generation 454 FLX Titanium pyrosequencing technology. The mean number of alleles per locus ranged from 2 to 4. Observed and expected heterozygosity ranged from 0.00 to 1.0 and 0.00 to 0.64, respectively. From 10 SSR markers developed for G. pumila, 9 markers are promising candidates for analyzing genetic variation within or between natural populations of G. pumila and other species from the same genus.

【 授权许可】

CC BY