Response of Bacterial Communities upon Application of Different Innovative Organic Fertilizers in a Greenhouse Experiment Using Low-Nutrient Soil Cultivated with Cynodon dactylon

Soil Systems

Peserico, Lisanna¹  Zanardo, Marina²  Sablok, Gaurav³  Meneghesso, Andrea⁴  Rosselli, Riccardo⁵  Altissimo, Adriano⁶  Engel, Marion⁷  Stevanato, Piergiorgio⁸ 

[1] Author to whom correspondence should be addressed;Department of Agronomy, Food, Natural Resources, Animals, and Environment—DAFNAE, University of Padova, Viale dell’Università 16, 35020 Legnaro (Padova), Italy;Department of Biodiversity and Molecular Ecology, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, San Michele all’Adige, 38010 Trento, Italy;Department of Biology, University of Padova, Via Ugo Bassi 58b, 35131 Padova, Italy;Division de Microbiología, Universidad Miguel Hernandez, 03202 San Juan de Alicante, Spain;Landlab Studio Associato, Via Quintarello 12A, 36050 Quinto Vicentino (Vicenza), Italy;Research Unit Scientific Computing, Helmholtz Zentrum München, Ingolstädter Landstraße 1, 85764 Neuherberg, Germany;Research Unit for Comparative Microbiome Analysis, Helmholtz Zentrum München, Ingolstädter Landstraße 1, 85764 Neuherberg, Germany

关键词: organic fertilizers;    bacterial communities;    16S amplicons;   

DOI  :  10.3390/soilsystems2030052

学科分类：土壤学

来源: mdpi

【 摘 要 】

Assessing the response of microbial communities to nutrient inputs in man-managed soils is of primary importance to understand the impact on ecosystem services provided by the soil microbiome. In this study, a low-nutrient soil was supplemented with seven different innovative fertilizers including matrixes of plant, animal, fungal or synthetic origin, and dosed to deliver the same amount of nitrogen. Growth of a potted grass crop (Cynodon dactylon) was recorded and the fertilizers were scored by the plant yield obtained in a greenhouse study. Soil was sampled at 9 and 58 days after the addition and bacterial community composition was analyzed after soil DNA extraction through pyrosequencing of 16S rDNA gene amplicons. Over 900 bacterial genera were detected, belonging to 21 described and 19 candidate phyla. In spite of the equal dose of nitrogen delivered, specific groups were fostered by given fertilizers; in particular marked effects on some phyla were displayed by a yeast-based fertilizer, which was also most effective in plant productivity. The main shifts were observed shortly after the fertilizer application, followed by a gradual stabilization of the equilibrium and by a rise in community evenness.

【 授权许可】

CC BY