Determination of serum bone-specific alkaline phosphatase isoenzyme activity in captive Asian elephants (Elephas maximus) using an agarose gel electrophoresis method

The Journal of Veterinary Medical Science

Norio YAMAGISHI¹  Rurika ONOMI²  Kazuya TAKEHANA³  Kaoru HATATE⁴ 

[1] Department of Veterinary Sciences, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8550, Japan;Graduate School of Animal and Veterinary Sciences and Agriculture, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8550, Japan;Ichihara Elephant Kingdom Zoological Park, Ichihara, Chiba 290-0521, Japan;United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan

关键词: agarose gel electrophoresis (AGE);    Asian elephants (Elephas maximus);    bone markers;    bone-specific alkaline phosphatase isoenzyme;   

DOI  :  10.1292/jvms.18-0688

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

The bone-specific alkaline phosphatase (ALP) isoenzyme activity was measured in 51 serum samples from four captive Asian elephants (Elephas maximus) using a conventional method with wheat germ lectin precipitation and a commercial agarose gel electrophoresis (AGE) kit; the isoenzymes were designated as bone-specific ALP (BAP) and ALP isoenzyme 3 (ALP3), respectively. This study examined the suitability of the AGE kit for analyzing blood biochemistry in Asian elephants. The serum ALP3 and BAP activities were strongly positively correlated and met the evaluation criteria for agreement using Bland-Altman analysis. The results indicate that the AGE kit can be used to examine the blood biochemistry in Asian elephants instead of the conventional method.

【 授权许可】

Unknown