Selection, isolation, and identification of fungi for bioherbicide production

Revista de microbiologia

Souza, Angélica Rossana Castro de¹  Baldoni, Daiana Bortoluzzi² 

[1] Universidade Federal de Santa Maria, Santa Maria, Brazil;Universidade Federal do Pampa, SantAna do Livramento, Brazil

关键词: Bioprospecting;    Diaporthe;    Bioproduct;    Liquid fermentation.;   

DOI  :  10.1016/j.bjm.2016.09.004

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

Production of a bioherbicide for biological control of weeds requires a series of steps, from selection of a suitable microbial strain to final formulation. Thus, this study aimed to select fungi for production of secondary metabolites with herbicidal activity using biological resources of the Brazilian Pampa biome. Phytopathogenic fungi were isolated from infected tissues of weeds in the Pampa biome. A liquid synthetic culture medium was used for production of metabolites. The phytotoxicity of fungal metabolites was assessed via biological tests using the plant Cucumis sativus L., and the most promising strain was identified by molecular analysis. Thirty-nine fungi were isolated, and 28 presented some phytotoxic symptoms against the target plant. Fungus VP51 belonging to the genus Diaporthe showed the most pronounced herbicidal activity. The Brazilian Pampa biome is a potential resource for the development of new and sustainable chemical compounds for modern agriculture.

【 授权许可】

CC BY-NC