Comparative study of nanostructured carriers of calcium phosphate and magnesium phosphate loaded with SRT1720 for the protection of H2O2-induced senescent endothelium

American Journal of Translational Research

Yi-Qin Shi¹  Rui-Lin Li²  Zhao-Yang Lu³  Xue-Lian Wang⁴  Zhi-Xiao Su⁵  Bing-Bing Ning⁶ 

[1] Clinical Research Unit, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Kongjiang Road 1665, Shanghai 200092, China;Department of Cardiology, Tenth Peoples Hospital of Tongji University, Yanchang Road 301, Shanghai 200072, China;Department of Nephrology, Zhongshan Hospital, Fudan University, Fenglin Road 180, Shanghai 200032, China;Division of Pulmonary and Critical Care Medicine, Department of Medicine, School of Medicine, University of Maryland, 20 Penn Street, HSF-2, Room #S112, Baltimore 21201, MD, USA;Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Meilong Road 130, Shanghai 200237, China;State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China

关键词: Nanostucture;    calcium phosphate;    magnesium phosphate;    SRT1720;    endothelial cell senescence;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

Nanostructured calcium phosphate (CaP) and magnesium phosphate (MgP) are promising for the application as the nanocarriers in drug delivery. However, the difference between CaP and MgP nanocarriers in drug delivery is rarely investigated. In this work, we comparatively investigated nanostructured CaP, MgP and calcium magnesium phosphate (CMP) for the delivery of SRT1720, which is a silent information regulator (SIRT1) specific activator with pro-angiogenic and anti-aging properties in response to hydrogen peroxide (H2O2)-induced endothelial senescence. The protection of SRT1720-loaded CaP nanospheres, MgP nanosheets and CMP microspheres on the H2O2-induced senescent endothelium was examined by using human umbilical vein endothelial cells (HUVECs), demonstrating the improved cell viability, anti-aging, tube formation and migration. In addition, the SRT1720-loaded CaP nanospheres, MgP nanosheets and CMP microspheres can rescue the impaired angiogenic potential of HUVECs via activation of Akt/eNOS/VEGF pathway. The SRT1720-loaded MgP nanosheets and CMP microspheres have a similar protective effect compared with the pure SRT1720, while the SRT1720-loaded CaP nanospheres decrease the protective capability of SRT1720. These results lead us to figure out both MgP nanosheets and CMP microspheres are suitable and effective delivery for SRT1720 and this system can be further applied in vivo treatment.

【 授权许可】

CC BY-NC