Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA

Revista de microbiologia

Franciscatto, Laura G.¹  Santos, Carla R.² 

[1] Universidade Feevale, Novo Hamburgo, Brasil;Universidade Luterana do Brasil, Canoas, Brasil

关键词: HPV;    cervical cancer;    FTA elute;    molecular detection.;   

DOI  :  10.1590/S1517-83822012000100047

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

This study aimed to evaluate the use of the FTA elute cardTM impregnated with cervicovaginal sample directly in the PCR amplification for detection of HPV-DNA. The results were compared to a reference technique. This method was more efficient than the protocol indicated by the manufacturer, identifying 91.7% against 54.2% of the positive samples.

【 授权许可】

CC BY-NC