Revista de microbiologia | |
Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome | |
Castro, Alessandra M.M.G. de1  Lima, Michele dos S.2  Martins, Maira de S.N.3  | |
[1] Complexo Educacional Faculdade Metropolitanas Unidas, São Paulo, Brazil;Coordenadoria de Defesa Agropecuária do Estado de São Paulo, São Paulo, Brazil;Instituto Biológico, São Paulo, Brazil | |
关键词: Ovine herpesvirus type 2 (OvHV-2); Histopathology; Qualitative PCR; Quantitative PCR; Phylogenetic analysis.; | |
DOI : 10.1016/j.bjm.2016.10.021 | |
学科分类:农业科学(综合) | |
来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology | |
【 摘 要 】
Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) were optimized and validated. The qualitative polymerase chain reaction (qualitative PCR) analytical sensitivity was 101 DNA copies/µL and found 4.8% (14/290) positive for OvHV-2. The quantitative polymerase chain reaction (qPCR) analytical sensitivity was 100 DNA copy/µL and 5.9% (17/290) positivity, with 47.1% (8/17) of the positive samples presenting histological evidence of non-purulent meningo-encephalitis. The qualitative PCR products (422 bp of the ORF75 region) were sequenced and submitted to phylogenetic analysis. Identity matrices showed 100% similarity in OvHV-2 samples obtained in this study and those recovered from GenBank, corroborating other studies.
【 授权许可】
CC BY-NC
【 预 览 】
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