Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome

Revista de microbiologia

Castro, Alessandra M.M.G. de¹  Lima, Michele dos S.²  Martins, Maira de S.N.³ 

[1] Complexo Educacional Faculdade Metropolitanas Unidas, São Paulo, Brazil;Coordenadoria de Defesa Agropecuária do Estado de São Paulo, São Paulo, Brazil;Instituto Biológico, São Paulo, Brazil

关键词: Ovine herpesvirus type 2 (OvHV-2);    Histopathology;    Qualitative PCR;    Quantitative PCR;    Phylogenetic analysis.;   

DOI  :  10.1016/j.bjm.2016.10.021

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) were optimized and validated. The qualitative polymerase chain reaction (qualitative PCR) analytical sensitivity was 101 DNA copies/µL and found 4.8% (14/290) positive for OvHV-2. The quantitative polymerase chain reaction (qPCR) analytical sensitivity was 100 DNA copy/µL and 5.9% (17/290) positivity, with 47.1% (8/17) of the positive samples presenting histological evidence of non-purulent meningo-encephalitis. The qualitative PCR products (422 bp of the ORF75 region) were sequenced and submitted to phylogenetic analysis. Identity matrices showed 100% similarity in OvHV-2 samples obtained in this study and those recovered from GenBank, corroborating other studies.

【 授权许可】

CC BY-NC