System for the heterologous expression of NS1 protein of H9N2 avian influenza virus in the ciliate Tetrahymena thermophila

The Journal of Veterinary Medical Science

Hongshu CHI¹  Chunhua ZHU²  Cuiteng CHEN³  Xiaodong LIU⁴ 

[1]Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou 350013, China

[2]Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China

[3]Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China

[4]University of Chinese Academy of Sciences, Beijing 100049, China

关键词: avian influenza;    heterologous expression;    NS1 gene;    Tetrahymena;    virus;   

DOI  :  10.1292/jvms.18-0291

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Tetrahymena is commonly used as an alternative eukaryotic system for efficiently expressing heterologous genes. In this study, we inserted the non-structural (NS) 1 gene of avian influenza virus (AIV) into the shuttle vector pD5H8 and transformed conjugating T. thermophila with the recombinant plasmid pD5H8-NS1 by particle bombardment. Positive transformants were selected with paromomycin. We demonstrated that the NS1 protein could be expressed steadily following induction with cadmium in this Tetrahymena system. An enzyme-linked immunosorbent assay detection method was preliminary established using the expressed protein as coating antigens for serodiagnosis. This is the first study in which a Tetrahymena expression system was employed for the expression of the AIV NS1 protein, and it provides a good basis for the development of differential diagnostic kits and vaccines for the prevention and control of avian influenza.

【 授权许可】

Unknown