Sp1 is an important transcriptional regulation factor for forkhead box N1 in pig

Journal of genetics

DONG JIE ZHANG^1¹ 

[1] Institute of Animal Husbandry, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, People’s Republic of China^1

关键词: pig;    Foxn11 gene;    transcriptional regulation;    Sp1;   

DOI  :  

学科分类：生物科学（综合）

来源: Indian Academy of Sciences

【 摘 要 】

The transcription factor forkhead box N1 (Foxn1) plays an important role in the development and function of thymic epithelial cells (TECs) in vertebrates. However, the transcriptional regulation of Foxn1 is still unknown. A series of dual luciferase report vectors were constructed and their relative activities were also detected. The 5′-untranslated regions contains two cis-acting elements, Sp1 and GATA-1, as well as trans-acting elements between positions −332 to −438. Nevertheless, the relative activities of Foxn1 promoter were significantly increased in PK15 cells only when the Sp1 was overexpressed, suggesting that the Sp1 wasthe most important sequence for Foxn1 transcription activation in the pig. Regrettably, the exact trans-acting elements were not found.

【 授权许可】

CC BY