Molecular typing of Mycobacterium bovis isolates: a review

Revista de microbiologia

Ramos, Daniela Fernandes¹  Tavares, Lucas² 

[1] Universidade Federal de Pelotas, Pelotas, Brazil;Universidade Federal de Rio Grande, Rio Grande, Brazil

关键词: tuberculosis;    bovine;    diagnosis;    genotyping;    Mycobacterium bovis.;   

DOI  :  10.1590/S1517-83822014005000045

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

Mycobacterium bovis is the main causative agent of animal tuberculosis (TB) and it may cause TB in humans. Molecular typing of M. bovis isolates provides precise epidemiological data on issues of inter- or intra-herd transmission and wildlife reservoirs. Techniques used for typing M. bovis have evolved over the last 2 decades, and PCR-based methods such as spoligotyping and mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) have been extensively used. These techniques can provide epidemiological information about isolates of M. Bovis that may help control bovine TB by indicating possible links between diseased animals, detecting and sampling outbreaks, and even demonstrating cases of laboratory cross-contamination between samples. This review will focus on techniques used for the molecular typing of M. bovis and discuss their general aspects and applications.

【 授权许可】

CC BY-NC