Sample manipulation and data assembly for robust microcrystal synchrotron crystallography

IUCrJ

Fuchs, M.R.¹  Skinner, J.²  Berman, E.³  Shi, W.⁴  Guo, G.⁵ 

[1]Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA

[2]Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA

[3]Department of Physiology and Cellular Biophysics, Columbia University, New York, NY 10032, USA

[4]GM/CA@APS, X-ray Science Division, Advanced Photon Source, Argonne National Laboratory, Argonne, IL 60439, USA

[5]Photon Science Directorate, NSLS-II, Brookhaven National Laboratory, Upton, NY 11973, USA

关键词: MICROCRYSTALS;    MICRODIFFRACTION;    RADIATION DAMAGE;    DATA ANALYSIS;    MULTIPLE CRYSTALS;    X-RAY CRYSTALLOGRAPHY;    STRUCTURAL BIOLOGY;   

DOI  :  10.1107/S2052252518005389

学科分类：数学（综合）

来源: International Union of Crystallography

【 摘 要 】

With the recent developments in microcrystal handling, synchrotron microdiffraction beamline instrumentation and data analysis, microcrystal crystallo­graphy with crystal sizes of less than 10 µm is appealing at synchrotrons. However, challenges remain in sample manipulation and data assembly for robust microcrystal synchrotron crystallography. Here, the development of micro-sized polyimide well-mounts for the manipulation of microcrystals of a few micrometres in size and the implementation of a robust data-analysis method for the assembly of rotational microdiffraction data sets from many microcrystals are described. The method demonstrates that microcrystals may be routinely utilized for the acquisition and assembly of complete data sets from synchrotron microdiffraction beamlines.

【 授权许可】

CC BY