PREPARATIVE SEPARATION OF ISOQUINOLINE ALKALOIDS FROM PHELLODENDRI CORTEX BY PH-ZONE-REFINING COUNTER-CURRENT CHROMATOGRAPHY

Quimica nova

Tian, Shanming¹  Wang, Daijie² 

[1] Shandong University, Jinan, China;TCM Quality Control, Jinan, China

关键词: Phellodendri Cortex;    isoquinoline alkaloids;    palmatine;    berberine;    pH-Zone-Refining Counter-Current Chromatography.;   

DOI  :  10.21577/0100-4042.20170184

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

pH-Zone-refining counter-current chromatography (PZRCCC) was successfully used for preparative separation isoquinoline alkaloids from Phellodendri Cortex. Two target compounds of berberine and palmatine were completely resolved by a two-phase solvent system chloroform/methanol/water (4:2:2, v/v), with 10 mmol L-1 HCl in upper stationary phase and 10 mmol L-1 TEA in lower organic phase. From 2.0 g of the crude alkaloids, palmatine (448 mg, 95.1%) and berberine (159 mg, 95.4%) were obtained in one-step PZRCCC separation. The chemical structures were identified by ESI-MS and NMR data. The results indicated that PZRCCC separation is an excellent preparative separation method for isoquinoline alkaloids from Phellodendri Cortex.

【 授权许可】

CC BY