Identification and extensive analysis of inverted-duplicated HBV integration in a human hepatocellular carcinoma cell line

BMB Reports

Kwang Joong Kim^1¹  Jeong Bok^1² 

[1] Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health, Chungcheongbuk-do 363-951, Korea^2;Division of Structural and Functional Genomics, Center for Genome Science^1

关键词: Chromosome 11q13;   

DOI  :  

学科分类：生物化学/生物物理

来源: Korean Society for Biochemistry and Molecular Biology

【 摘 要 】

Hepatitis B virus (HBV) DNA is often integrated into hepatocellular carcinoma (HCC). Although the relationship between HBV integration and HCC development has been widely studied, the role of HBV integration in HCC development is still not completely understood. In the present study, we constructed a pooled BAC library of 9 established cell lines derived from HCC patients with HBV infections. By amplifying viral genes and superpooling of BAC clones, we identified 2 clones harboring integrated HBV DNA. Screening of host-virus junctions by repeated sequencing revealed an HBV DNA integration site on chromosome 11q13 in the SNU-886 cell line. The structure and rearrangement of integrated HBV DNA were extensively analyzed. An inverted duplicated structure, with fusion of at least 2 HBV DNA molecules in opposite orientations, was identified in the region. The gene expression of cancer-related genes increased near the viral integration site in HCC cell line SNU-886.

【 授权许可】

Unknown