An efficient SCNT technology for the establishment of personalized and public human pluripotent stem cell banks

BMB Reports

Jeoung Eun Lee^1¹  Jin Hee Eum^1²  Young Gie Chung^2³ 

[1] CHA Stem Cell Institute, CHA University, Seongnam 13488, Korea^1;Department of Biomedical Science, CHA University, Seongnam 13488, Korea^3;Research Institute for Stem Cell Research, CHA Health Systems, Los Angeles, CA 90036, USA^2

关键词: ESC derivation;   

DOI  :  

学科分类：生物化学/生物物理

来源: Korean Society for Biochemistry and Molecular Biology

【 摘 要 】

Although three different research groups have reported successful derivations of human somatic cell nuclear transfer-derived embryonic stem cell (SCNT-ESC) lines using fetal, neonatal and adult fibroblasts, the extremely poor development of cloned embryos has hindered its potential applications in regenerative medicine. Recently, however, our group discovered that the severe methylation of lysine 9 in Histone H3 in a human somatic cell genome was a major SCNT reprogramming barrier, and the overexpression of KDM4A, a H3K9me3 demethylase, significantly improved the blastocyst formation of SCNT embryos. In particular, by applying this new approach, we were able to produce multiple SCNT-ES cell lines using oocytes obtained from donors whose eggs previously failed to develop to the blastocyst stage. Moreover, the success rate was closer to 25%, which is comparable to that of IVF embryos, so that our new human SCNT method seems to be a practical approach to establishing a pluripotent stem cell bank for the general public as well as for individual patients.

【 授权许可】

Unknown