Fixed-target serial oscillation crystallography at room temperature

IUCrJ

Cook, M.J.¹  Besaw, J.E.²  Par-Labrosse, O.³  Wierman, J.L.⁴  Sarracini, A.⁵ 

[1] Department of Physics, Cornell University, Ithaca, NY 14853, USA;Departments of Biochemistry and Molecular Genetics, University of Toronto, Toronto, Ontario, Canada;Departments of Chemistry and Physics, University of Toronto, Toronto, ON Canada;MacCHESS, Cornell University, Ithaca, NY 14853, USA;Max Planck Institute for the Structure and Dynamics of Matter, Hamburg, Germany

关键词: FIXED-TARGET SERIAL OSCILLATION;    SERIAL CRYSTALLOGRAPHY;    OSCILLATIONS;    STRUCTURAL BIOLOGY;    RADIATION DAMAGE;    STRUCTURE DETERMINATION;    X-RAY CRYSTALLOGRAPHY;    STORAGE RINGS;   

DOI  :  10.1107/S2052252519001453

学科分类：数学（综合）

来源: International Union of Crystallography

【 摘 要 】

A fixed-target approach to high-throughput room-temperature serial synchrotron crystallography with oscillation is described. Patterned silicon chips with microwells provide high crystal-loading density with an extremely high hit rate. The microfocus, undulator-fed beamline at CHESS, which has compound refractive optics and a fast-framing detector, was built and optimized for this experiment. The high-throughput oscillation method described here collects 1–5° of data per crystal at room temperature with fast (10° s−1) oscillation rates and translation times, giving a crystal-data collection rate of 2.5 Hz. Partial datasets collected by the oscillation method at a storage-ring source provide more complete data per crystal than still images, dramatically lowering the total number of crystals needed for a complete dataset suitable for structure solution and refinement – up to two orders of magnitude fewer being required. Thus, this method is particularly well suited to instances where crystal quantities are low. It is demonstrated, through comparison of first and last oscillation images of two systems, that dose and the effects of radiation damage can be minimized through fast rotation and low angular sweeps for each crystal.

【 授权许可】

CC BY