Roles of Cyclin A, Myc, Jun and Ppm1l in tumourigenic transformation of NIH3T3 cell

Journal of genetics

LINGLING XI^1¹  JIHONG ZHANG^2²  CUIFANG CHANG^1³ 

[1]Department of Histology and Embryology, Xinxiang Medical University, Xinxiang 453007, People’s Republic of China^2

[2]Henan Provincial People’s Hospital, Zhengzhou 450000, People’s Republic of China^3

[3]State Key Laboratory Cultivation Base for Cell Differentiation Regulation, Henan Normal University, Xinxiang 453007, People’s Republic of China^1

关键词: tumourigenic transformation;    oncogenes;    tumour suppressor genes;    signalling transduction;    gene expression profile.;   

DOI  :  

学科分类：生物科学（综合）

来源: Indian Academy of Sciences

【 摘 要 】

To analyse the mechanism of tumourigenic transformation of NIH3T3 cells at the transcriptional level, we used cancerogen 3-methylcholanthrene (3-MCA) and cancerogenic substance phorbol-12-myristate-13-acetate (PMA) to transform NIH3T3 cells and the assessment of transformation was performed using Giemsa staining and methylcellulose colony formation assay. Changes in gene expression profile were detected by MouseGenome 430 2.0 microarray; and quantitative real-time polymerase chain reaction and Western blotting were used to verify the expression changes of mRNAs and proteins, respectively. With the aidof bioinformatics method, five signalling pathways were identified to participate in different stages of NIH3T3 cell transformation. Further, our study suggested that oncogenes Cyclin A, Myc, Jun and the tumour suppressor gene Ppm1l may play important roles in these pathways.

【 授权许可】

CC BY