Characteristics of Lignin Fractions from Dilute Acid Pretreated Switchgrass and Their Effect on Cellobiohydrolase from Trichoderma longibrachiatum

Frontiers in Energy Research

Yoo, Chang Geun¹  Yao, Lan²  Meng, Xianzhi³  Yang, Haitao⁴ 

[1] Department of Chemical and Biomolecular Engineering, The University of Tennessee, United States;Hubei Provincial Key Laboratory of Green Materials for Light Industry, Hubei University of Technology, China;Joint Institute for Biological Sciences, Biosciences Division, Oak Ridge National Laboratory (ORNL), United States;State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, China

关键词: characterization;    Lignin;    Dilute acid pretreatment;    Switchgrass;    Cellobiohydrolases I;   

DOI  :  10.3389/fenrg.2018.00001

学科分类：能源（综合）

来源: Frontiers

【 摘 要 】

To investigate the interactions between acid pretreated grass lignin and cellobiohydrolase, three different lignin fractions were isolated from dilute acid pretreated switchgrass by (i) ethanol extraction, followed by (ii) dioxane/H2O extraction, and (iii) cellulase treatment, respectively. Structural properties of each lignin fraction were elucidated by GPC, 13C-NMR and 2D-HSQC NMR analyses. The adsorptions of cellobiohydrolase to the isolated lignin fractions were also studied by Langmuir adsorption isotherms. Ethanol-extractable lignin fraction, mainly composed of syringyl (S) and guaiacyl (G) units, had the lowest molecular weight, while dioxane/H2O-extractable lignin fraction had the lowest S/G ratio with higher content of p-coumaric acid (pCA) unit. The residual lignin fraction after enzymatic treatment had the highest S/G ratio without hydroxyphenyl (H) unit. Strong associations were found between lignin properties such as lignin composition and S/G ratio and its non-productive enzyme adsorption factors including the maximum adsorption capacity and binding strength.

【 授权许可】

CC BY