Therapeutic potential of human umbilical cord derived mesenchymal stem cells on rat model of liver fibrosis

American Journal of Stem Cells

Ahmed Taher El-Serafi¹  Howayda Abdelall²  Sahar Mansour Greish³  Yasser Mohamed El-Wazir⁴  Mona Farouk Mansour⁵ 

[1]Department of Clinical and Experimental Medicine, Linkping University, Sweden

[2]Medical Biochemistry Department, Faculty of Medicine, Suez Canal University, Egypt

[3]Medical Science Department, School of Oral and Dental Medicine, Badr University in Cairo, Egypt

[4]Pathology Department, Faculty of Medicine, Suez Canal University, Egypt

[5]Physiology Department, Faculty of Medicine, Suez Canal University, Egypt

关键词: Mesenchymal stem cells;    CD34-ve cells;    hepatic regeneration;    liver fibrosis;    immune response;    cell therapy;   

DOI  :  

学科分类：分子生物学，细胞生物学和基因

来源: e-Century Publishing Corporation

【 摘 要 】

End-stage liver disease is a worldwide cause of morbidity and mortality, which is associated with a considerable economic burden. As the disease progresses, fibrosis will replace the hepatic architecture and compromise liver functions. The regenerative approach for the injured liver can provide a hope for these patients; however, it is still facing many challenges. In the current study, we aimed at (1) assessing hepatic regenerative capacity of mesenchymal stem cells, isolated from human umbilical cord blood (HMSCs), in a rat model of carbon-tetrachloride (CCL4) induced liver fibrosis, (2) comparing the therapeutic effects with other cell populations derived from umbilical cord blood and (3) evaluating the host response to the human-derived cells. Fifteen rats received either the whole mononuclear cell fraction (HMNCs), CD34-ve subpopulation or HMSCs. A fourth group did not receive any treatment and another group was left without induction of fibrosis as positive and negative controls. All groups that received cellular treatment showed homing of the human cells and improvement of the liver architecture and functional capacity. The groups received CD34-ve cells and HMSCs had the most efficient improvement in liver functions, microscopic regenerative markers and histological appearance while the least immune reaction was noted with HMSCs. HUCB-MSCs showed significant immunemodulatory effect on rat immune cells. This study can provide a clue about a simple and effective method for the management of fibrotic liver diseases.

【 授权许可】

CC BY-NC