Isolation and fusion of protoplasts from the phytopathogenic fungus Sclerotium rolfsii (Sacc.)

Revista de microbiologia

Hayat, Sikandar¹  Christias, Christos² 

[1] Fellow Alberta Research Council, Vegreville, Canada;University of Patras, Greece

关键词: Protoplast;    fusion;    Sclerotium rolfsii;    Pathogenicity and osmotic stabilizer.;   

DOI  :  10.1590/S1517-83822010000100035

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

Sclerotium rolfsii (Sacc.) is a serious plant pathogenic fungus and lacks perfect (basidial) stage in production. Protoplast fusion technology was employed to reconstruct fusants from this fungus. Two strains designated as A and R were used. Maximum protoplast yields of 3.8x105 /g mycelia and 2.8x105 /g mycelia were formed in strains A and R respectively. Osmotic stabilizer sucrose 1M gave maximum yield. Lysing enzyme at the rate of 15mg/ml was found best for yield. Fusion of protoplasts from strains A and R was carried out in fusion media containing PEG 4000 30% (w/v) with 0.2mM CaCl2. Four fusants F1, F2, F3 and F4 were recovered. Morphological, physiological and pathogenic characters of fusants were compared with parent strains on carrots, beans and tomato.

【 授权许可】

CC BY-NC