Investigation of the Relation between Follicular Atresia and Granulosa Cells in Terms of Cell Death Mechanisms in Premature Ovarian Failure Model

Proceedings

Uluer, Elgin Turkoz¹  Akogullari, Damla²  Vatansever, H. Seda³ 

[1] Department of Histology & Embryology, Faculty of Medicine, Manisa Celal Bayar University, Manisa 45030, Turkey;Department of Histology & Embryology, Institute of Medical Sciences, Manisa Celal Bayar University, Manisa 45030, Turkey;Experimental Health Sciences Research Center, Near East University, Nicosia 99138, Cyprus

关键词: Premature Ovarian Failure;    cyclophosphamide;    follicular atresia;    apoptosis;    autophagy;    paraptosis;   

DOI  :  10.3390/proceedings2251529

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

Premature Ovarian Failure; is characterized by the dysfunction or early depletion of ovarian reserves due to follicular loss in the ovary in women under age of 40. POF is the important cause of infertility and its etiology is still not clearly understood. Investigation of cell death mechanisms (CDM) that play a role in the follicular atresia (FA) triggered by excessive loss of granulosa cells (GCs) that provide metabolic support for oocyte and follicle development in the ovary will help to understand POF etiology. It was known that apoptosis and autophagy play a role in FA. Recent studies have shown that paraptosis, associated with endoplasmic reticulum stress (ERS), also exist in FA. POF model was established in C57BL/6 female mice by CTX and it was confirmed by increased follicle stimulating hormone (FSH), luteal hormone (LH) and decreased estradiol (E2) blood levels and follicle count. According to the results of the immunohistochemistry (IHC) cell death markers were significantly more expressed than control (C) and sham (S) groups in the POF model. In addition, more apoptotic cells were observed in the POF group compared to C and S in the TUNEL analysis. In consequence of this study apoptosis and autophagy as well as paraptosis play a role in the FA leading to POF, will help to develop new treatment protocols.

【 授权许可】

CC BY