Stem Cell Research | |
Meticulous optimization of cardiomyocyte yields in a 3-stage continuous integrated agitation bioprocess - ScienceDirect | |
Sherwin Ting^11  Alan Lam^12  | |
[1] Bioprocessing Technology Institute, A*STAR (Agency for Science, Technology and Research), Singapore 138668, Singapore^1;National Heart Centre Singapore, Singapore 169609, Singapore^2 | |
关键词: Human pluripotent stem cells; Microcarriers; Spinner platform; Cardiomyocytes differentiation; Lactate purification; Ascorbic acid; | |
DOI : 10.1016/j.scr.2018.07.020 | |
学科分类:生物科学(综合) | |
来源: Elsevier | |
【 摘 要 】
Human pluripotent stem cells (hPSCs) can be a renewable source for generating cardiomyocyte (CM) for treating myocardial infraction. In our previous publication, we described an integrated microcarrier-based wave reactor process for the expansion and differentiation of hPSCs to CMs on a rocker based platform. However, this platform is limited in terms of linear scalability and CMs purity. The present study describes ways to overcome these limitations by the use of a stirred scalable platform and incorporation of an additional lactate based purification step which increases CM purity.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201910252421415ZK.pdf | 2288KB | download |