Meticulous optimization of cardiomyocyte yields in a 3-stage continuous integrated agitation bioprocess - ScienceDirect

Stem Cell Research

Sherwin Ting^1¹  Alan Lam^1² 

[1] Bioprocessing Technology Institute, A*STAR (Agency for Science, Technology and Research), Singapore 138668, Singapore^1;National Heart Centre Singapore, Singapore 169609, Singapore^2

关键词: Human pluripotent stem cells;    Microcarriers;    Spinner platform;    Cardiomyocytes differentiation;    Lactate purification;    Ascorbic acid;   

DOI  :  10.1016/j.scr.2018.07.020

学科分类：生物科学（综合）

来源: Elsevier

【 摘 要 】

Human pluripotent stem cells (hPSCs) can be a renewable source for generating cardiomyocyte (CM) for treating myocardial infraction. In our previous publication, we described an integrated microcarrier-based wave reactor process for the expansion and differentiation of hPSCs to CMs on a rocker based platform. However, this platform is limited in terms of linear scalability and CMs purity. The present study describes ways to overcome these limitations by the use of a stirred scalable platform and incorporation of an additional lactate based purification step which increases CM purity.

【 授权许可】

Unknown