Human corneal endothelial cell transplantation using nanocomposite gel sheet in bullous keratopathy

American Journal of Stem Cells

Kazutoshi Haraguchi¹  Rajappa Senthilkumar³  Periasamy Parikumar⁴  Samuel JK Abraham⁵ 

[1] Department of Applied Molecular Chemistry, College of Industrial Technology, Nihon University, Chiba, Japan;Edogawa Evolutionary Laboratory of Science (EELS), Edogawa Hospital, Tokyo, Japan;The Fujio-Eiji Academic Terrain (FEAT), Nichi-In Centre for Regenerative Medicine (NCRM), Chennai, Tamil Nadu, India;The Light Eye Hospital, Dharmapuri, Tamil Nadu, India;The Mary-Yoshio Translational Hexagon (MYTH), Nichi-In Centre for Regenerative Medicine (NCRM), Chennai, Tamil Nadu, India;Yamanashi University, School of Medicine, Chuo, Japan

关键词: Corneal dystrophy;    cell transplantation;    nanocomposites;    hydrogel;   

DOI  :  

学科分类：分子生物学，细胞生物学和基因

来源: e-Century Publishing Corporation

【 摘 要 】

Transplantation of in vitro expanded human corneal endothelial precursors (HCEP) cells using a nanocomposite (D25-NC) gel sheet as supporting material in bovine’s cornea has been earlier reported. Herein we report the transplantation of HCEP cells derived from a cadaver donor cornea to three patients using the NC gel sheet. In three patients with bullous keratopathy, one after cataract surgery, one after trauma and another in the corneal graft, earlier performed for congenital corneal dystrophy, not amenable to medical management HCEP cells isolated from a human cadaver donor cornea in vitro expanded using a thermoreversible gelation polymer (TGP) for 26 days were divided into three equal portions and 1.6 × 105 HCEP cells were injected on to the endothelium of the affected eye in each patient using the D25-NC gel sheet as a supporting material. The sheets were removed after three days. The bullae in the cornea disappeared by the 3rd-11th post-operative day in all the three patients. Visual acuity improved from Perception of light (PL)+/Projection of rays (PR)+ to Hand movements (HM)+ in one of the patients by post-operative day 3 which was maintained at 18 months follow-up. At 18 months follow-up, in another patient the visual acuity had improved from HM+ to 6/60 while in the third patient, visual acuity remained HM+ as it was prior to HCEP transplantation. There were no adverse effects during the follow-up in any of the patients.

【 授权许可】

CC BY-NC