High fat diet activates adult mouse lung stem cells and accelerates several aging-induced effects - ScienceDirect

Stem Cell Research

Mari Ozaki^1¹  Ahmed E. Hegab^1² 

[1] Department of Histology, Faculty of Medicine, Assiut University, Assiut, Egypt^2;Division of Pulmonary Medicine, Department of Medicine, Keio University School of Medicine, Tokyo, Japan^1

关键词: AT2;    alveolar type-2;    BALT;    bronchus-associated lymphoid tissues;    CR;    Calorie restriction;    CFE;    colony forming efficiency;    H&;    E;    hematoxylin and eosin;    HFD;    High fat diet, used as (HF) in figures and table 7;    LNEP;    lineage negative epithelial progenitor;    LCD;    low calorie diet;    MTECs;    mouse tracheal epithelial cells;    ROS;    reactive oxygen species;    SDHA;    Succinate dehydrogenase complex, subunit A;    SD;    standard diet;    Sftpc/SP-C;    surfactant protein C;    WLCs;    whole lung cells;    VALT;    vascular-associated lymphoid tissues;    High fat diet;    Aging;    Alveolar cells;    Lung stem cells;    Mitochondria;    Calorie restriction;   

DOI  :  10.1016/j.scr.2018.10.006

学科分类：生物科学（综合）

来源: Elsevier

【 摘 要 】

High fat diet (HFD) decreases the lifespan of mice, and is a risk factor for several human diseases. Here, we investigated the effects of a HFD on lung epithelial and stem cells and its interaction with aging. Young and old mice were fed with either a standard diet (SD) or a HFD then their trachea and lung were examined for histological changes, inflammation, and mitochondrial function. Their stem cell function was examined using the in vitro organoid/colony forming efficiency (CFE) assay. Aging reduced the number of tracheal basal and alveolar type-2 (AT2) cells. HFD significantly increased the number of AT2 cells. Aging also caused a significant increase in lung inflammation, and HFD caused a similar increase, in young mice. Aging reduced mitochondrial mass and function, and increased reactive oxygen species. In young mice, HFD caused mitochondrial changes similar to the aging-induced changes. Organoid culture of tracheal and lung epithelial cells collected from both young and old HFD-fed mice showed higher CFE compared to SD-fed mice. Switching the HFD to low calorie/fat diet (LCD) efficiently reversed several of the HFD-induced effects. Thus, HFD induces several histological, inflammatory, and functional changes in the lung, and exacerbates the aging-induced lung inflammation and mitochondrial deterioration. LCD can reverse many of the HFD-induced effects.

【 授权许可】

Unknown