Enhancement of HIV-1 Tat fusion protein transduction efficiency by bog blueberry anthocyanins

BMB Reports

Eun Jeong Sohn^1¹  Dae Won Kim^1²  Soon Sung Lim^4³  Sun Hwa Lee^1⁴  Duk Soo Kim^2⁵  Mi Jin Kim^1⁶  Tae Cheon Kang^3⁷  Hoon Jae Jeong^1⁸ 

[1] Department of Anatomy &^4;Department of Anatomy, College of Medicine, Soonchunhyang University, Cheonan 330-090^3;Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine, Seoul 138-736, Korea^8;Department of Biomedical Science &^1;Department of Food Science and Nutrition &^6;Neurobiology, College of Medicine, Hallym University, Chunchon 200-702^5;RIC Center, Hallym University, Chunchon 200-702^7;Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 200-702^2

关键词: Bog blueberry anthocyanins;   

DOI  :  

学科分类：生物化学/生物物理

来源: Korean Society for Biochemistry and Molecular Biology

【 摘 要 】

Though protein transduction domains (PTDs) are well known for the delivery of exogenous therapeutic proteins into living cells, the overall low efficiency of transduction is a serious obstacle. We investigated the effect of bog blueberry anthocyanins (BBA) on protein transduction efficiency and found that BBA enhanced the transduction efficiencies of Tat-SOD fusion protein into HeLa cells and mice skin. The enzymatic activities in the cells and skin tissue in the presence of BBA were markedly increased compared to controls. Further, BBA did not demonstrate any cell toxicity at various concentrations. Although the mechanism is not fully understood, we suggest that BBA might alter the conformation of the membrane, which would indicate that BBA can be used as a protein transduction enhancer for the efficient delivery of therapeutic proteins for a variety of disorders.

【 授权许可】

Unknown