Frequency Assessment of OXA-10 and PER β-Lactamase Genes and Determination of Minimum Inhibitory Concentration in Klebsiella Strains Isolated from Urinary Tract Infections

Jundishapur Journal of Microbiology

Milad Shahini Shams Abadi¹  Farshad Kakian²  Behnam Zamanzad³ 

[1] Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran;Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;Social Health Determinants Research Center and Epidemiology and Biostatistics, School of Health, Shahrekord University of Medical Sciences, Shahrekord, Iran

关键词: Frequency Assessment of OXA-10 and PER β-Lactamase Genes and Determination of Minimum Inhibitory Concentration in Klebsiella Strains Isolated from Urinary Tract Infections;    Klebsiella;    Minimum Inhibitory Concentration;    Extended Spectrum β-Lactamases;    OXA-10;    PER Gene;   

DOI  :  10.5812/jjm.65500

学科分类：微生物学和免疫学

来源: Jundishapur Journal of Microbiology

【 摘 要 】

Klebsiella is one of the Enterobacteriaceae family that causes infections such as pneumonia, urinary tract infections (UTI), and meningitis. Klebsiella strains are capable of producing enzymes that can degrade the third-generation of cephalosporins known as broad-spectrum beta-lactamase enzymes. The resistance of Klebsiella strains to β-lactam antibiotics is related to the presence of β-lactamase genes.In this study, 90 isolates of Klebsiella were isolated from two inpatient and outpatient groups, each of them was 45 isolates, which were collected from patients with urinary tract infection in educational hospitals of Shahrekord. The isolates were identified using phenotypic agar diffusion, disc phenotypic confirmation tests, and E-test of extended-spectrum beta-lactamase (ESBL). The PCR molecular method was used to diagnose and determine the strains containing broad-spectrum β-lactamases.Thirty (66%) inpatients and 8 (17.8%) outpatients had broad-spectrum β-lactamase enzymes. The frequency of β-lactamase OXA-10 genes and PER in inpatients were 90% and 33%, respectively and also in outpatients were 50% and 12.5%, respectively.This study showed that the prevalence of isolated Klebsiella producing broad-spectrum β-lactamases is higher in inpatients in comparison to outpatients. Therefore, the rapid and accurate identification of bacteria and their resistance genes in clinical microbiology labs are highly recommended.

【 授权许可】

CC BY-NC