Regional characteristics of Lactobacillus plantarum group strains isolated from two kinds of Japanese post-fermented teas, Ishizuchi-kurocha and Awa-bancha

Bioscience of Microbiota, Food and Health

Sayaka NAKAMURA¹  Masanori HORIE²  Atsumi TADA³  Hiroaki SATO⁴ 

[1] Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-11, Inada-Cho, Obihiro, Hokkaido 080-8555, Japan;Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14, Hayashi-Cho, Takamatsu, Kagawa, Japan;Professor Emeritus of Kagawa University, 232-3 Donyu, Wakayama, Wakayama 640-8432, Japan;Reserch Institute for Sustainable Chemistry, AIST, 1-1-1 Higashi, Tsukuba, Japan

关键词: Lactobacillus plantarum;    postfermented tea;    ribosomal protein;    Lactobacillus pentosus;   

DOI  :  10.12938/bmfh.18-005

学科分类：生物科学（综合）

来源: Nihon Bifizusukin Senta / Japan Bifidus Foundation

【 摘 要 】

Properties of Lactobacillus plantarum group strains isolated from two kinds of Japanese post-fermented teas, Ishizuchi-kurocha and Awa-bancha, were compared. Although lactic acid bacteria isolated from the fermented teas were identified as L. plantarum via homology comparison of 16S ribosomal RNA gene sequences, classification of L. plantarum based on ribosomal proteins showed that the strains isolated from Ishizuchi-kurocha and Awa-bancha were different. According to classification by the ribosomal protein typing, Ishizuchi-kurocha-derived strains belong to the same group as L. plantarum subsp. plantarum JCM 1149T. Awa-bancha-derived strains were assigned to a different group. This pattern was also applicable to strains isolated more than 10 years ago. A further analysis based on recA and a dnaK gene showed that Awa-bancha-derived strains were closely related to L. pentosus. The interactions with cultured cells were different between strain JCM 1149T and the Ishizuchi-kurocha-derived strains. The Ishizuchi-kurocha-derived strains showed strong adhesion to Caco-2 cells. In contrast, strain JCM 1149T and the Awa-bancha-derived strains hardly adhered to Caco-2 cells. According to the ribosomal protein typing, sugar utilization, and interaction with Caco-2 cells, although these properties were dependent on the strain strictly speaking, the L. plantarum group strains in this study can be subdivided into two groups: (1) type strain JCM 1149T and Ishizuchi-kurocha-derived strains and (2) Awa-bancha-derived strains. A regionally unique microorganism may persist in each traditional fermented drink.

【 授权许可】

CC BY