期刊论文详细信息
Revista de microbiologia
Invasin gimB found in a bovine intestinal Escherichia coli with an adherent and invasive profile
Matter, Letícia B.1 
[1] Universidade Federal de Santa Maria, Santa Maria, BR
关键词: gimB;    adherence;    invasiveness;    zoonotic potential;    livestock;    companion animals.;   
DOI  :  10.1590/S1517-838246320140621
学科分类:农业科学(综合)
来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology
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【 摘 要 】

The invasin gimB (genetic island associated with human newborn meningitis) is usually found in ExPEC (Extraintestinal Pathogenic Escherichia coli) such as UPEC (uropathogenic E. coli), NMEC (neonatal meningitis E. coli) and APEC (avian pathogenic E. coli). In NMEC, gimB is associated with the invasion process of the host cells. Due to the importance of E. coli as a zoonotic agent and the scarce information about the frequency of gimB-carrying strains in different animal species, the aim of this study was to investigate the presence of gimB in isolates from bovine, swine, canine and feline clinical samples. PCR was conducted on 196 isolates and the identity of the amplicons was confirmed by sequencing. Of the samples tested, only E. coli SB278/94 from a bovine specimen was positive (1/47) for gimB, which represents 2.1% of the bovine isolates. The ability of SB278/94 to adhere to and invade eukaryotic cells was confirmed by adherence and gentamicin-protection assays using HeLa cells. This is the first study that investigates for gimB in bovine, canine and feline E. coli isolates and shows E. coli from the intestinal-bovine samples harboring gimB.

【 授权许可】

CC BY-NC   

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