Identification of Natural Products as Novel PI3Kβ Inhibitors Through Pharmacophore‐based Virtual Screening

Bulletin of the Korean Chemical Society

Hyun Cheol Chung¹  Xuemei Jin²  Tae Soo Kim³  JungNyoung Heo⁴  Woosun Kwon⁵ 

[1]Bioinformatics & Molecular Design Research Center (BMDRC) Yonsei University Seoul 03722 Korea

[2]Department of Biotechnology Yonsei University Seoul 03722 Korea

[3]Korea Research Institute of Chemical Technology Daejeon 34114 Republic of Korea

[4]SongDang Institute for Cancer Research Cancer Metastasis Research Center, Medical Oncology, Yonsei Cancer Center, Yonsei University College of Medicine Seoul 03722 Korea

[5]SongDang Institute for Cancer Research Cancer Metastasis Research Center, Yonsei University College of Medicine Seoul 03722 Korea

关键词: Phosphatidylinositol 3‐;    kinase beta;    Pharmacophore‐;    based virtual screening;    Molecular docking;    Molecular dynamics simulation;    Phosphatidylinositol 3‐;    kinase beta inhibitors;   

DOI  :  10.1002/bkcs.11382

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Phosphatidylinositol 3‐kinase beta (PI3Kβ) is the dominant isoform of PI3K and has been implicated in thrombosis as well as phosphatase and tensin homologue‐loss‐induced tumorigenesis. PI3Kβ has been considered to be an attractive target for anticancer drug discovery, and several PI3Kβ inhibitors have progressed into clinical trials. Here, we disclose the discovery of two natural products (PBY‐0002 and PBY‐0006) that have inhibitory effects on PI3Kβ. These two natural products were identified through pharmacophore‐based virtual screening, molecular docking, and a molecular dynamics simulation. Furthermore, an in vitro assay against human gastric cancer cell lines revealed that these two compounds showed anticancer activity. To identify the binding modes of PBY‐0002 and PBY‐0006 further, we performed a systematical investigation with comparison to the binding mode of GSK2636711, which is a known PI3Kβ inhibitor. The results demonstrated that PBY‐0002 and PBY‐0006 were tightly embedded into the ATP‐binding site via hydrogen bonds and π‐cation interactions. These two natural products can provide a promising starting point for the rational design of potent analogs with inhibitory activity against PI3Kβ.

【 授权许可】

Unknown