Isolation and characterization of canine parvovirus type 2c (CPV-2c) from symptomatic puppies

Revista de microbiologia

Pérez, R¹  Puentes, R²  Eliopulos, N³ 

[1] Empresa Brasileira de Pesquisa Agropecuária, Concórdia, Brasil;UdelaR, Montevideo, Uruguay;Universidade Federal de Pelotas, Pelotas, Brasil

关键词: Canine Parvovirus 2c;    Isolation;    Molecular characterization;    South America.;   

DOI  :  10.1590/S1517-83822012000300022

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

Canine parvovirus type 2 (CPV-2) is a leading cause of diarrhea in puppies in several parts of the world. In this study CPV-2 was detected and recovered from puppies showing clinical disease from Montevideo, Uruguay. Samples were processed and used to infect CRFK and MDCK cells in order to isolate the virus. Out of twelve, two samples were positive for CPV-2. A genomic region of 583 bp was amplified and the molecular characterization was performed by sequencing, phylogenetic analysis and Restriction Fragment Length Polymorphism (RFLP). Two isolated viruses (UY1 and UY2) were CPV-2c-like viruses. The comparison between the cytophatic effect (CPE) of CPV-2 (vaccinal virus) and CPV-2c (isolated virus) on primary canine cells cultures and on CRFK line cells, demonstrated that CPV-2c is less citopathogenic in CRFK than in primary cultures. Our study represents the first report on isolation and characterization of canine parvovirus type 2c (CPV-2c) in cell cultures from South American dogs.

【 授权许可】

CC BY-NC