On the Home Front: Specialised Reference Testing for Dengue in the Australasian Region

Tropical Medicine and Infectious Disease

McMahon, Jamie¹  Taylor, Carmel²  Gunn, Wendy³  Mackay, Ian M.⁴  Pyke, Alyssa T.⁵ 

[1] Author to whom correspondence should be addressed;Child Health Research Centre, The University of Queensland, South Brisbane, QLD 4101, Australia;Institute of Environmental Science and Research Limited, Wallaceville, 5018 Upper Hutt, New Zealand;Metro North Public Health Unit, Metro North Hospital and Health Service, Queensland Health, Windsor, QLD 4030, Australia;Public Health Virology Laboratory, Forensic and Scientific Services, Coopers Plains, QLD 4108, Australia

关键词: dengue;    dengue virus;    arbovirus;    chikungunya virus;    Zika virus;    reference laboratory;    real-time RT-PCR;    ELISA;    microsphere immunoassay;    luminex;    genotyping;    whole genome sequencing;   

DOI  :  10.3390/tropicalmed3030075

学科分类：传染病学

来源: mdpi

【 摘 要 】

Reference laboratories are vital for disease control and interpreting the complexities and impact of emerging pathogens. The role of these centralized facilities extends beyond routine screening capabilities to provide rapid, specific, and accurate diagnoses, advanced data analysis, consultation services, and sophisticated disease surveillance and monitoring. Within the Australasian region, the Public Health Virology Laboratory (PHV), Forensic and Scientific Services, Department of Health, Queensland Government, Australia, and the Institute of Environmental Science and Research Limited (ESR), New Zealand (NZ) perform specialised reference testing and surveillance for dengue viruses (DENVs) and other emerging arthropod-borne viruses (arboviruses), including chikungunya virus (CHIKV) and Zika virus (ZIKV). With a focus on DENV, we review the reference testing performed by PHV (2005 to 2017) and ESR (2008 to 2017). We also describe how the evolution and expansion of reference-based methodologies and the adoption of new technologies have provided the critical elements of preparedness and early detection that complement frontline public health control efforts and limit the spread of arboviruses within Australasia.

【 授权许可】

CC BY