期刊论文详细信息
American Journal of Translational Research
Calcitriol exerts a mineralization-inductive effect comparable to that of vitamin C in cultured human periodontium cells
Chun-Chieh Wang1  Yi-Fang Huang2  Cheng-Cheng Chiang3  Tzung-Hai Yen4  Hsiang-Hsi Hong5  E-Wen Huang6  Adrienne Hong7 
[1]California Northstate University, College of Medicine, Elk Grove, CA, United States
[2]Department of General Dentistry, Chang Gung Memorial Hospital, Linkou, Taiwan
[3]Department of Materials Science and Engineering, National Chiao Tung University, Hsinchu, Taiwan
[4]Department of Nephrology, Clinical Poison Center, Kidney Research Center, Center for Tissue Engineering, Chang Gung Memorial Hospital and Chang Gung University, Linkou, Taiwan
[5]Department of Periodontics, Chang Gung Memorial Hospital and Chang Gung University, Linkou, Taiwan
[6]National Synchrotron Radiation Research Center, Hsinchu, Taiwan
[7]School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan
关键词: Cultured human periodontium cells;    vitamin C;    calcitriol;    synchrotron transmission X-ray microscope;    mineralization;   
DOI  :  
Subject:61.1
来源: e-Century Publishing Corporation
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【 摘 要 】
This study inspected whether calcitriol could exert a mineralization-inductive effect comparable to that of vitamin C in cultured human periodontium cells (hPDCs). The mRNA expression of the mineralization-related biomarkers core-binding factor subunit alpha-1 (Cbfa1), collagen 1 α1 (Col-I), alkaline phosphatase (ALP), osteopontin (OPN), bone sialoprotein (BSP), osteocalcin (OCN), vitamin D receptor (VDR), cementum protein 1 (CEMP-1), cementum attachment protein (CAP), interleukin 6 (IL-6), transforming growth factor-β1 (TGF-β1) and osteoprotegerin (OPG) was surveyed after incubation of hPDCs with vitamin C and calcitriol for 2 weeks. Translational expression information from ALP activity and CEMP-1 and CAP immunofluorescence assays was acquired from hPDCs at the second and third weeks. Extracellular calcifications were confirmed by von Kossa staining, Alizarin Red staining and synchrotron transmission X-ray microscopy (TXM) at the fourth and fifth weeks. It was found that both vitamin C and calcitriol not only increased mineralization-related mRNA fold-changes but also enhanced ALP activity, CEMP-1 immunofluorescence, von Kossa and Alizarin Red staining and TXM-associated calcifications. Generally, 10-8 M calcitriol displayed greater mineralization significance than 10-7 M calcitriol in the assays tested. However, vitamin C stimulated lower Cbfa1, Col-1, ALP, OPN, BSP, OCN, VDR, CEMP-1 and IL-6 mRNA fold-changes than 10-8 M calcitriol. Finally, TXM analysis indicated that a 10-8 M calcitriol treatment stimulated greater calcifications than vitamin C treatment. Therefore, the analytical results confirmed the osteo-inductive potential of vitamin C in cultured hPDCs. In contrast, 10-8 M calcitriol could potentially function as a substitute because it stimulates a greater mineralization effect than vitamin C or 10-7 M calcitriol.
【 授权许可】

CC BY-NC   

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