An improved microsatellite panel to assess genetic variability of the Italian smooth newt (Lissotriton vulgaris meridionalis)

Journal of genetics

GIORGIA GALLIANI^1¹  VINCENZO BUONO^1² 

[1] Area per la Genetica della Conservazione, Istituto per la Protezione e la Ricerca Ambientale (ISPRA), Via Cà Fornacetta 9, 40064 Ozzano dell’Emilia, BO, Italy^2;Dipartimento di Scienze, Università Roma Tre, Viale Marconi 446, 00146 Rome, Italy^1

关键词: conservation genetics;    drinking troughs;    amphibians;    population structure;   

DOI  :  

学科分类：生物科学（综合）

来源: Indian Academy of Sciences

【 摘 要 】

Habitat loss and fragmentation are threatening amphibians by increasing population isolation. However, artificial waterbodies created for livestockmay contrast this phenomenon by providing surrogate habitats for amphibians.Here, we performed a genetic study on an amphibian species, Lissotriton vulgaris meridionalis, in a rural area in central Italy where natural wetlandsare disappearing and drinking troughs for cattle watering are widespread. Specifically, we tested a panel of microsatellite markers to identify a suitable tool for addressing conservation genetic issues of this species that is undergoing severe local decline. Twelve of the 20 tested loci produced reliable amplifications and were polymorphic. Three distinct units with a low level of gene flow weredistinguished and the population genetic structuring overlapped with geographic distribution. Such loci will be useful to assess the genetic diversity of the species across multiscale levels for its management and conservation.

【 授权许可】

CC BY