Comparative analysis of rosaceous genomes and the reconstruction of a putative ancestral genome for the family

BMC Evolutionary Biology

Ross Crowhurst¹  Massimo Pindo²  Daniel J Sargent³  Jill Bushakra⁴  Eudald Illa⁵  Alessandro Cestaro⁶  Elena Lopez Girona⁷ 

[1] Department of Horticulture and Crop Science, The Ohio State University/Ohio Agricultural Research and Development Center, Wooster, USA;Department of Horticulture, Michigan State University, East Lansing, USA;East Malling Research, Kent, UK;IASMA Research and Innovation Centre, Foundation Edmund Mach, San Michele allAdige, Italy;IRTA, Centre de Recerca en Agrigenmica CSIC-IRTA-UAB, Cabrils (Barcelona), Spain;The New Zealand Institute for Plant & Food Research Limited, Mt Albert Research Centre, Auckland, New Zealand;The New Zealand Institute for Plant & Food Research Limited, Palmerston North Research Centre, Palmerston North, New Zealand

关键词: Linkage Group;    High Resolution Melting;    Ancestral Genome;    Syntenic Block;    Translocation Event;   

DOI  :  10.1186/1471-2148-11-9

学科分类：生物科学（综合）

来源: BioMed Central

【 摘 要 】

Comparative genome mapping studies in Rosaceae have been conducted until now by aligning genetic maps within the same genus, or closely related genera and using a limited number of common markers. The growing body of genomics resources and sequence data for both Prunus and Fragaria permits detailed comparisons between these genera and the recently released Malus × domestica genome sequence. We generated a comparative analysis using 806 molecular markers that are anchored genetically to the Prunus and/or Fragaria reference maps, and physically to the Malus genome sequence. Markers in common for Malus and Prunus, and Malus and Fragaria, respectively were 784 and 148. The correspondence between marker positions was high and conserved syntenic blocks were identified among the three genera in the Rosaceae. We reconstructed a proposed ancestral genome for the Rosaceae. A genome containing nine chromosomes is the most likely candidate for the ancestral Rosaceae progenitor. The number of chromosomal translocations observed between the three genera investigated was low. However, the number of inversions identified among Malus and Prunus was much higher than any reported genome comparisons in plants, suggesting that small inversions have played an important role in the evolution of these two genera or of the Rosaceae.

【 授权许可】

CC BY