Progesterone modulates microtubule dynamics and epiboly progression during zebrafish gastrulation

Developmental biology

Mario Ringler^1¹  Stephanie Eckerle^1²  Virginie Lecaudey^1,2³ 

[1] BIOSS - Centre for Biological Signalling Studies, Albertstrasse 19, 79104 Freiburg, Germany^2;Developmental Biology, Institute Biology I, Faculty of Biology, Albert-Ludwigs-University Freiburg, Hauptstrasse 1, D-79104 Freiburg, Germany^1;Zentrum für Biosystemanalyse, Albert-Ludwigs-University Freiburg, Habsburgerstr. 49, D-79104 Freiburg, Germany^3

关键词: DCL;    deep cell layer;    EMTB;    Ensconsin microtubule-binding domain;    EVL;    enveloping layer;    hpf;    hours post fertilization;    MAP;    microtubule associated protein;    MBT;    midblastula transition;    MTOC;    microtubule organizing center;    P4;    progesterone;    P5;    pregnenolone;    SEM;    standard error of the mean;    YCL;    yolk cytoplasmic layer;    YSL;    yolk syncytial layer;    Microtubule;    Microtubule dynamics;    Gastrulation;    Epiboly;    Cytoskeleton;    Zebrafish;    Development;    Progesterone;    Pregnenolone;   

DOI  :  10.1016/j.ydbio.2017.12.016

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Control of microtubule dynamics is crucial for cell migration. We analyzed regulation of microtubule network dynamics in the zebrafish yolk cell during epiboly, the earliest coordinated gastrulation movement. We labeled microtubules with EMTB-3GFP and EB3-mCherry to visualize and measure microtubule dynamics by TIRF microscopy live imaging. Yolk cell microtubules dynamics is temporally modulated during epiboly progression. We used maternal zygotic Pou5f3 mutant (MZspg) embryos, which develop strong distortions of microtubule network organization and epiboly retardation, to investigate genetic control of microtubule dynamics. In MZspg embryos, microtubule plus-end growth tracks move slower and are less straight compared to wild-type. MZspg embryos have altered steroidogenic enzyme expression, resulting in increased pregnenolone and reduced progesterone levels. We show that progesterone positively affects microtubule plus-end growth and track straightness. Progesterone may thus act as a non-cell-autonomous regulator of microtubule dynamics across the large yolk cell, and may adjust differing demands on microtubule dynamics and stability during initiation and progression phases of epiboly.

【 授权许可】

CC BY