Identification and comparative analysis of the miRNA expression profiles from four tissues of Micropterus salmoides using deep sequencing

Genomics

Yong Huang^2¹  Jun Xie^1,3²  Wangbao Gong^1³ 

[1] College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China^2;Guangdong Ecological Remediation of Aquaculture Pollution Research Center, Guangzhou 510380, China^3;Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China^1

关键词: Micropterus salmoides;    miRNA;    Deep sequencing;    Expression;   

DOI  :  10.1016/j.ygeno.2018.09.017

学科分类：医学（综合）

来源: Academic Press

【 摘 要 】

In the present study, four small RNA libraries were constructed from an M. salmoides population and sequenced using deep sequencing technology. A total of 9,888,822; 8,519,365; 20,566,198; and 15,762,254 raw reads representing 666,097; 755,711; 978,923; and 840,175 unique sequences were obtained from the spleen, liver, kidney, and muscle libraries, respectively. As a result, 509 known miRNAs belonging to 143 families and 1157 novel miRNAs were identified. The miRNAs displayed diverse expression levels among the four libraries, among which most of the known miRNAs were expressed at higher levels than the novel miRNAs. Furthermore, stem-loop qRT-PCR was applied to validate and profile the expression of the differentially expressed miRNAs in the four different tissues, which revealed that some miRNAs showed tissue specific expression. The identification of miRNAs in M. salmoides will provide new information and enhance our understanding of the functions of miRNAs in regulating biological processes.

【 授权许可】

CC BY