Molecular pathology of total knee arthroplasty instability defined by RNA-seq

Genomics

William H. Trousdale^1¹  Christopher G. Salib^1²  Eric A. Lewallen^1,2³ 

[1] Department of Biochemistry & Molecular Biology, Mayo Clinic, Rochester, MN, United States^3;Department of Biological Sciences, Hampton University, Hampton, VA, United States^2;Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, United States^1

关键词: Total knee arthroplasty;    Flexion instability;    Revision total knee arthroplasty;    Cell biology;    Molecular genetics;   

DOI  :  10.1016/j.ygeno.2017.11.001

学科分类：医学（综合）

来源: Academic Press

【 摘 要 】

Total knee arthroplasty (TKA) is a durable and reliable procedure to alleviate pain and improve joint function. However, failures related to flexion instability sometimes occur. The goal of this study was to define biological differences between tissues from patients with and without flexion instability of the knee after TKA. Human knee joint capsule tissues were collected at the time of primary or revision TKAs and analyzed by RT-qPCR and RNA-seq, revealing novel patterns of differential gene expression between the two groups. Interestingly, genes related to collagen production and extracellular matrix (ECM) degradation were higher in samples from patients with flexion instability. Partitioned clustering analyses further emphasized differential gene expression patterns between sample types that may help guide clinical interpretations of this complication. Future efforts to disentangle the effects of physical and biological (e.g., transcriptomic modifications) risk factors will aid in further characterizing and avoiding flexion instability after TKA.

【 授权许可】

CC BY