miR-328 prevents renal fibrogenesis by directly targeting TGF-β2

Bratislava Medical Journal

W. He¹ 

关键词: renal fibrosis;    miRNA;    miR-328;    TGF-β;    ECM;    EMT;   

DOI  :  10.4149/BLL_2018_079

学科分类：医学（综合）

来源: AEPress, s.r.o.

【 摘 要 】

MicroRNAs have been shown to be crucial in the post-transcriptional regulation of various cellular biological functions. They also play important roles in various human diseases. In renal fibrosis, aberrant miRNA expression is observed. However, little is known about the roles of miR-328 in renal fibrogenesis and its regulating mechanisms. In the present study, we investigated the miR-328 expression profile in TGF-β1-induced renal fibrogenesis cell model using qRT-PCR. The results showed that TGF-β1 could induce EMT expression and fibrogenesis and significantly down-regulated the expression of miR-328. Through bioinformatical analysis, we identified that TGF-β2 was a direct target gene of miR-328. Luciferase reporter assay was conducted to further confirm this finding. The results also demonstrated that miR-328 mimics transfection could significantly upregulate miR-328 expression. Furthermore, upregulation of miR-328 could further repress the expression of TGF-β2 and ECM proteins. In conclusion, this study demonstrated that miR-328 could prevent renal fibrogenesis by directly targeting TGF-β2. Our findings suggested that elevated renal miR-328 levels might be a novel therapeutic strategy for treating renal fibrosis (Fig. 4, Ref. 36).

【 授权许可】

CC BY