Honey activity on the proliferation and migration of oral squamous cell carcinoma: In vitro and bioinformatic analysis

Scientific Research and Essays

Eliane Macedo Sobrinho Santos¹ 

关键词: Aroeira honey;    Myracrodruon urundeuva;    wild honey;    Apis mellifera;    therapeutic;    Jequitinhonha River Valley.;   

DOI  :  10.5897/SRE2018.6590

学科分类：社会科学、人文和艺术（综合）

来源: Academic Journals

【 摘 要 】

This study aimed to investigate, in oral squamous carcinoma cells (OSCC), the antineoplastic effect of Apis mellifera honey produced from Aroeira and wildflower, in the region of Jequitinhonha River Valley, Brazil. Furthermore, we investigate the molecular mechanism of honey performance through bioinformatic analysis. Honey and OSCC-related gene list were screened using GeneCards. The online tool STRING was used to identify the potential interaction networks of protein products of these genes. The genes with the highest weighted number of links (WNL) were called leader genes. GO and KEGG pathway analysis was performed to classify the genes. We use a search tool STITCH for interactions of chemicals-proteins. The chemical characterization of honey and its antiproliferative and antimigratory effects in SCC-9 cell lines was evaluated in vitro. The analysis of the interaction network obtained from the STRING database indicated by the following leader genes: TNF, IL6 and HDAC1. GO analysis for the honey, and OSCC-related proteins revealed that leader genes-regulated cancers genes were markedly enriched in some functions. Through the chemical characterization, the bioactive classes: steroids/triterpenoids, flavonoids, saponins, alkaloids, and tannins were identified. Polyphenols act in activating CASP3 and TP53 and inhibit the action of DNMT1. The honey produced from Aroeira and wildflower reduced proliferative phenotype and migration of OSCC cells.

【 授权许可】

CC BY