Hypertonic solution-induced preconditioning reduces inflammation and mortality rate

Journal of Inflammation

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[1] 0000 0004 1937 0722, grid.11899.38, Laboratório de Investigação Médica – LIM 51, Faculdade de Medicina, Universidade de São Paulo (FMUSP), São Paulo, Brazil;0000 0004 1937 0722, grid.11899.38, Laboratório de Investigação Médica – LIM 51, Faculdade de Medicina, Universidade de São Paulo (FMUSP), São Paulo, Brazil;0000 0004 1937 0722, grid.11899.38, Emergências Clínicas do Departamento de Clínica Médica da Faculdade de Medicina da Universidade de São Paulo, Av Dr Arnaldo 455, room 3189, 01246-903, São Paulo, CEP, Brazil;

关键词: Sepsis;    Inflammation;    Preconditioning;    Hypertonic solution;    Tolerance;   

DOI  :  10.1186/s12950-019-0220-4

来源: publisher

【 摘 要 】

BackgroundDysregulated inflammatory response is common cause of organ damage in critical care patients. Preconditioning/tolerance is a strategy to prevent exacerbated inflammation. The aim of this study is to analyze hypertonic saline 7.5% as a potential inducer of preconditioning that protect from a lethal dose of LPS and modulates systemic inflammatory profile in mice.MethodsMale Balb/C mice received intravenous (i.v.) injections of Hypertonic solution (NaCl 7.5%) (0.8 ml) for 3 days, on day 8th was challenged with LPS 15 mg/kg. Controls with Saline 0.9%, urea and sorbitol were performed. Microarray of mRNA expression was analyzed from HS versus saline from macrophages to identified the pathways activated by HS.ResultsHS preconditioning reduced mortality after LPS injection as well reduced the cytokines release in plasma of the animals challenged by LPS. In order to check how HS induces a preconditioning state we measured plasma cytokines after each HS infusion. Repeated HS injections induced a state of preconditioning that reprograms the inflammatory response, resulting in reduced inflammatory cytokine production. A microarray of mRNA demonstrated that Hypertonic solution increased the expression of several genes in special Mapkbp1 and Atf3.Conclusionhypertonic solution induces preconditioning/tolerance reducing mortality and inflammatory response after LPS challenge.

【 授权许可】

CC BY