Epidermal cell turnover across tight junctions based on Kelvin's tetrakaidecahedron cell shape

eLife

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[1] Department of Biochemistry, Keio University School of Medicine, Tokyo, Japan;Suematsu Gas Biology Project, Exploratory Research for Advanced Technology, Japan Science and Technology, Tokyo, Japan;Department of Bioengineering, Faculty of Engineering, Imperial College London, London, United Kingdom;Department of Dermatology, Keio University School of Medicine, Tokyo, Japan;Department of Dermatology, Keio University School of Medicine, Tokyo, Japan;KOSÉ Corporation, Tokyo, Japan;Department of Dermatology, Keio University School of Medicine, Tokyo, Japan;Nerima General Hospital, Tokyo, Japan;Department of Dermatology, Keio University School of Medicine, Tokyo, Japan;RIKEN Center for Integrative Medical Sciences, Yokohama, Japan;Division of Cell Structure, National Institute for Physiological Sciences, Okazaki, Japan;Department of Physiological Sciences, SOKENDAI (The Graduate University for Advanced Studies), Okazaki, Japan;

关键词: epidermal barrier;    tissue homeostasis;    tight junction;    Mouse;   

DOI  :  10.7554/eLife.19593

来源: publisher

【 摘 要 】

10.7554/eLife.19593.001In multicellular organisms, cells adopt various shapes, from flattened sheets of endothelium to dendritic neurons, that allow the cells to function effectively. Here, we elucidated the unique shape of cells in the cornified stratified epithelia of the mammalian epidermis that allows them to achieve homeostasis of the tight junction (TJ) barrier. Using intimate in vivo 3D imaging, we found that the basic shape of TJ-bearing cells is a flattened Kelvin's tetrakaidecahedron (f-TKD), an optimal shape for filling space. In vivo live imaging further elucidated the dynamic replacement of TJs on the edges of f-TKD cells that enables the TJ-bearing cells to translocate across the TJ barrier. We propose a spatiotemporal orchestration model of f-TKD cell turnover, where in the classic context of 'form follows function', cell shape provides a fundamental basis for the barrier homeostasis and physical strength of cornified stratified epithelia.DOI: http://dx.doi.org/10.7554/eLife.19593.001

【 授权许可】

CC BY