Serum metabolomic alterations in Beagle dogs experimentally infected with Toxocara canis

Parasites & Vectors

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[1] 0000 0001 0018 8988, grid.454892.6, State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, Gansu, People’s Republic of China;0000 0004 1936 8868, grid.4563.4, Faculty of Medicine and Health Sciences, School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, LE12 5RD, Loughborough, UK;National Seed Center of Experimental Dogs, Guangzhou General Pharmaceutical Research Institute Co. Ltd, 510240, Guangzhou, Guangdong, People’s Republic of China;grid.257160.7, Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Hunan Engineering Technology Research Center of Veterinary Drugs, College of Veterinary Medicine, Hunan Agricultural University, 410128, Changsha, Hunan, People’s Republic of China;grid.257160.7, Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Hunan Engineering Technology Research Center of Veterinary Drugs, College of Veterinary Medicine, Hunan Agricultural University, 410128, Changsha, Hunan, People’s Republic of China;0000 0001 0018 8988, grid.454892.6, State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, Gansu, People’s Republic of China;

关键词: Toxocara canis;    Toxocariasis;    Beagle dog;    Serum;    Metabolomic;    LC-MS/MS;   

DOI  :  10.1186/s13071-019-3703-5

来源: publisher

【 摘 要 】

BackgroundToxocara canis, a globally distributed roundworm, can cause debilitating disease in dogs and humans; however, little is known about the metabolomic response of the hosts to T. canis infection. There is an increasing need to understand the metabolic mechanisms underlying the pathogenesis of T. canis infection in dogs. Here, we examined the metabolomic changes in Beagle dogsʼ serum following T. canis infection using LC-MS/MS.ResultsThe metabolic profiles of Beagle dogsʼ serum were determined at 12 h, 24 h, 10 d and 36 d after oral infection with 300 infectious T. canis eggs by LC-MS/MS. We tested whether the T. canis-associated differentially abundant metabolites could distinguish the serum of infected dogs from controls, as measured by the area under the receiver operating characteristic (ROC) curve (AUC). The differentially expressed metabolites were further evaluated by principal components analysis and pathway enrichment analysis. A total of 5756 and 5299 ions were detected in ESI+ and ESI− mode, respectively. ROC curve analysis revealed nine and five metabolite markers, at 12 hpi and 24 hpi to 36 dpi, respectively, with potential diagnostic value for toxocariasis. The levels of taurocholate, estradiol, prostaglandins and leukotriene were significantly changed. Primary bile acid biosynthesis pathway, steroid hormone biosynthesis pathway and biosynthesis of unsaturated fatty acids pathway were significantly altered by T. canis infection.ConclusionsThese findings show that T. canis infection can induce several changes in the dog serum metabolome and that the metabolic signature associated with T. canis infection in dogs has potential for toxocariasis diagnosis.

【 授权许可】

CC BY