Transcriptome analysis of Polygonatum cyrtonema Hua: identification of genes involved in polysaccharide biosynthesis

Plant Methods

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[1] 0000 0000 9490 772X, grid.186775.a, Anhui Medical University, 230032, Hefei, China;0000 0004 1757 8247, grid.252251.3, Anhui University of Chinese Medicine and Anhui Academy of Chinese Medicine, 230038, Hefei, China;0000 0004 0632 3409, grid.410318.f, State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China;0000 0004 1757 8247, grid.252251.3, Anhui University of Chinese Medicine and Anhui Academy of Chinese Medicine, 230038, Hefei, China;0000 0004 1757 8247, grid.252251.3, Clinical College of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, China;0000 0004 1757 8247, grid.252251.3, Anhui University of Chinese Medicine and Anhui Academy of Chinese Medicine, 230038, Hefei, China;0000 0004 1757 8247, grid.252251.3, Key Laboratory of Xin’an Medicine, Ministry of Education, Anhui University of Chinese Medicine, 230038, Hefei, China;0000 0004 1757 8247, grid.252251.3, Anhui University of Chinese Medicine and Anhui Academy of Chinese Medicine, 230038, Hefei, China;0000 0004 1757 8247, grid.252251.3, Key Laboratory of Xin’an Medicine, Ministry of Education, Anhui University of Chinese Medicine, 230038, Hefei, China;Synergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement, 230012, Hefei, China;0000 0004 1757 8247, grid.252251.3, Anhui University of Chinese Medicine and Anhui Academy of Chinese Medicine, 230038, Hefei, China;Synergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement, 230012, Hefei, China;

关键词: Polygonatum cyrtonema;    RNA-Seq;    Transcriptome;    Polysaccharides;    Metabolic pathways;    Gene expression;   

DOI  :  10.1186/s13007-019-0441-9

来源: publisher

【 摘 要 】

BackgroundPolygonatum cyrtonema Hua (P. cyrtonema) is one of the most important herbs in traditional Chinese medicine. Polysaccharides in P. cyrtonema plants comprise a class of important secondary metabolites and exhibit a broad range of pharmacological functions.ResultsIn order to identify genes involved in polysaccharide biosynthesis, we performed RNA sequencing analysis of leaf, root, and rhizome tissues of P. cyrtonema. A total of 164,573 unigenes were obtained by assembling transcripts from all three tissues and 86,063 of these were annotated in public databases. Differentially expressed genes (DEGs) were determined based on expression profile analysis, and DEG levels in rhizome tissues were then compared with their counterparts in leaf and root tissues. This analysis revealed numerous genes that were either up-regulated or uniquely expressed in the rhizome. Multiple genes encoding important enzymes, such as UDP glycosyltransferases (UGTs), or transcription factors involved in polysaccharide biosynthesis were identified and further analyzed, while a few genes encoding key enzymes were experimentally validated using quantitative real-time PCR.ConclusionOur results substantially expand the public transcriptome dataset of P. cyrtonema and provide valuable clues for the identification of candidate genes involved in metabolic pathways.

【 授权许可】

CC BY