Drosophila ML-DmD17-c3 cells respond robustly to Dpp and exhibit complex transcriptional feedback on BMP signaling components

BMC Developmental Biology

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[1] 0000 0000 9159 4457, grid.411023.5, Center for Vision Research and Department of Ophthalmology, Upstate Medical University, NRB-4610, 505 Irving Ave, 13210, Syracuse, NY, USA;0000 0000 9159 4457, grid.411023.5, Center for Vision Research and Department of Ophthalmology, Upstate Medical University, NRB-4610, 505 Irving Ave, 13210, Syracuse, NY, USA;0000 0000 9159 4457, grid.411023.5, Department of Biochemistry and Molecular Biology, Upstate Medical University, Syracuse, NY, USA;0000 0000 9159 4457, grid.411023.5, Department of Neuroscience and Physiology, Upstate Medical University, Syracuse, NY, USA;0000 0000 9159 4457, grid.411023.5, Center for Vision Research and Department of Ophthalmology, Upstate Medical University, NRB-4610, 505 Irving Ave, 13210, Syracuse, NY, USA;0000 0000 9159 4457, grid.411023.5, Department of Biochemistry and Molecular Biology, Upstate Medical University, Syracuse, NY, USA;grid.417307.6, Current Address: Department of Surgical Pathology, Yale-New Haven Hospital, New Haven, CT, USA;

关键词: Bone morphogenetic protein (BMP);    Decapentaplegic (Dpp);    Schneider (S2) cells;    ML-DmD17-c3 cells;    Phospho-mad (pMad);    Daughters against Dpp (Dad);    Punt (Put);    Thickveins (Tkv);    Wishful thinking (Wit);    Saxophone (Sax);   

DOI  :  10.1186/s12861-019-0181-0

来源: publisher

【 摘 要 】

BackgroundBMP signaling is involved in myriad metazoan developmental processes, and study of this pathway in Drosophila has contributed greatly to our understanding of its molecular and genetic mechanisms. These studies have benefited not only from Drosophila’s advanced genetic tools, but from complimentary in vitro culture systems. However, the commonly-used S2 cell line is not intrinsically sensitive to the major BMP ligand Dpp and must therefore be augmented with exogenous pathway components for most experiments.ResultsHerein we identify and characterize the responses of Drosophila ML-DmD17-c3 cells, which are sensitive to Dpp stimulation and exhibit characteristic regulation of BMP target genes including Dad and brk. Dpp signaling in ML-DmD17-c3 cells is primarily mediated by the receptors Put and Tkv, with additional contributions from Wit and Sax. Furthermore, we report complex regulatory feedback on core pathway genes in this system.ConclusionsNative ML-DmD17-c3 cells exhibit robust transcriptional responses to BMP pathway induction. We propose that ML-DmD17-c3 cells are well-suited for future BMP pathway analyses.

【 授权许可】

CC BY