Glutamine protects against oxidative stress injury through inhibiting the activation of PI3K/Akt signaling pathway in parkinsonian cell model

Environmental Health and Preventive Medicine

  ^{1    1    1    1    1    2} 

[1] 0000 0004 0646 966X, grid.449637.b, Innovation Research Center of Acupuncture and Medicine, Shaanxi University of Chinese Medicine, Qindu District, Xianyang, Shaanxi, China;Shaanxi Key Laboratory of Acupuncture and Medicine, Qindu District, Xianyang, Shaanxi, China;0000 0004 1765 1045, grid.410745.3, Nanjing University of Chinese Medicine, 138 Xianlin Avenue, Nanjing, China;0000 0004 0646 966X, grid.449637.b, College of Acupuncture and Moxibustion, Shaanxi University of Chinese Medicine, Qindu District, Xianyang, China;

关键词: Parkinson’s disease;    Oxidative stress;    Glutamine;    PC12;    PI3K/Akt;   

DOI  :  10.1186/s12199-018-0757-5

来源: publisher

【 摘 要 】

BackgroundParkinson’s disease is a neurodegenerative disorder, and recent studies suggested that oxidative stress contributes to the degeneration of dopamine cell in Parkinson’s disease. Glutamine also has a positive role in reducing oxidative stress damage. In this study, we hypothesized that glutamine offers protection against oxidative stress injury in 1-methyl-4-phenylpyridinium (MPP+)-induced Parkinson’s disease cell model.MethodsMPP+ was used to induce PD models in PC12 cells and classified into control, M0 (MPP+), G0 (glutamine), and M0+G0 groups. CCK-8 and AO/EB staining assays were used to examine cell proliferation and apoptosis, respectively. Western blotting was applied to examine the protein expression of PI3K, P-Akt, Akt, P-mTOR, and mTOR.ResultsWe showed that glutamine suppressed cytotoxicity induced by MPP+ in PC12 cells. MPP+ decreased the superoxide dismutase and glutathione peroxidase activity and increased the malondialdehyde content, which were restored by glutamine. Moreover, MPP+ increased the expression of PI3K, P-Akt, Akt, P-mTOR, and mTOR, which were inhibited by glutamine. And the antioxidant capacity of glutamine on PC12 cells could be improved by LY294002 and inhibited by IGF-1.ConclusionThese results suggest that glutamine strengthens the antioxidant capacity in PC12 cells induced by MPP+ through inhibiting the activation of the PI3K/Akt signaling pathway. The effects of glutamine should be investigated and the protective mechanism of glutamine in PD must be explored in future studies.

【 授权许可】

CC BY