DNA barcoding a unique avifauna: an important tool for evolution, systematics and conservation

BMC Evolutionary Biology

  ^{1    2    3    3    4    5    6    6    6    7    8    9    1,10} 

[1] 0000 0000 9224 802X, grid.431295.8, Unitec Institute of Technology, Auckland, New Zealand;0000 0001 0126 6191, grid.412970.9, Institute for Animal Ecology and Cell Biology, University of Veterinary Medicine Hannover Foundation, Bünteweg 17d, D-30559, Hannover, Germany;0000 0001 2197 9375, grid.421647.2, Department of Natural History, Royal Ontario Museum, 100 Queen’s Park, M5S 2C6, Toronto, Ontario, Canada;0000 0001 2157 2938, grid.17063.33, Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcox Street, M5S 3B2, Toronto, Ontario, Canada;0000 0001 2197 9375, grid.421647.2, Department of Natural History, Royal Ontario Museum, 100 Queen’s Park, M5S 2C6, Toronto, Ontario, Canada;0000 0001 2157 2938, grid.17063.33, Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcox Street, M5S 3B2, Toronto, Ontario, Canada;0000 0004 0473 9646, grid.42327.30, Present address: Laboratory Research Project Manager, The Hospital for Sick Children, Toronto, Ontario, Canada;0000 0001 2261 2209, grid.464524.5, Canterbury Museum, Rolleston Ave, 8001, Christchurch, New Zealand;0000 0004 0372 3343, grid.9654.e, School of Biological Sciences, University of Auckland, Private Bag 92019, 1142, Auckland, New Zealand;0000 0004 0437 5432, grid.1022.1, Environmental Futures Research Institute, Griffith University, 170 Kessels Road, 4111, Brisbane, Queensland, Australia;0000 0004 0500 6540, grid.436717.0, Molecular Biology Sciences Department, Museum Victoria, GPO Box 666, 3001, Melbourne, Victoria, Australia;0000000121532610, grid.1031.3, Present address: Graduate School, Southern Cross University, Lismore, New South Wales, Australia;0000000121532610, grid.1031.3, National Marine Science Centre, Southern Cross University, Coffs Harbour, New South Wales, Australia;Associate Emeritus, Auckland War Memorial Museum, Private Bag 92018, 1142, Auckland, New Zealand;

关键词: New Zealand birds;    c;    COI;    Specimen identification;    Conservation;    DNA barcodes;   

DOI  :  10.1186/s12862-019-1346-y

来源: publisher

【 摘 要 】

BackgroundDNA barcoding utilises a standardised region of the cytochrome c oxidase I (COI) gene to identify specimens to the species level. It has proven to be an effective tool for identification of avian samples. The unique island avifauna of New Zealand is taxonomically and evolutionarily distinct. We analysed COI sequence data in order to determine if DNA barcoding could accurately identify New Zealand birds.ResultsWe sequenced 928 specimens from 180 species. Additional Genbank sequences expanded the dataset to 1416 sequences from 211 of the estimated 236 New Zealand species. Furthermore, to improve the assessment of genetic variation in non-endemic species, and to assess the overall accuracy of our approach, sequences from 404 specimens collected outside of New Zealand were also included in our analyses. Of the 191 species represented by multiple sequences, 88.5% could be successfully identified by their DNA barcodes. This is likely a conservative estimate of the power of DNA barcoding in New Zealand, given our extensive geographic sampling. The majority of the 13 groups that could not be distinguished contain recently diverged taxa, indicating incomplete lineage sorting and in some cases hybridisation. In contrast, 16 species showed evidence of distinct intra-species lineages, some of these corresponding to recognised subspecies. For species identification purposes a character-based method was more successful than distance and phylogenetic tree-based methods.ConclusionsDNA barcodes accurately identify most New Zealand bird species. However, low levels of COI sequence divergence in some recently diverged taxa limit the identification power of DNA barcoding. A small number of currently recognised species would benefit from further systematic investigations. The reference database and analysis presented will provide valuable insights into the evolution, systematics and conservation of New Zealand birds.

【 授权许可】

CC BY