Using Next-Generation Sequencing Transcriptomics To Determine Markers of Post-traumatic Symptoms: Preliminary Findings from a Post-deployment Cohort of Soldiers

G3: Genes, Genomes, Genetics

article

Cathy Boscarino¹  Thomas Nalpathamkalam²  Giovanna Pellecchia²  Weili Li²  Bhooma Thiruvahindrapuram²  Daniele Merico³ 

[1] Operational Health and Performance Section, Defence Research & Development Canada - Toronto Research Center, Toronto ON Canada;The Centre for Applied Genomics, The Hospital for Sick Children, Peter Gilgan Centre for Research and Learning, Toronto ON Canada;Deep Genomics Inc., Toronto ON Canada

关键词: Post-traumatic stress;    expression profiling;    next-generation sequencing;    soldiers;   

DOI  :  10.1534/g3.118.200516

学科分类：社会科学、人文和艺术（综合）

来源: Genetics Society of America

【 摘 要 】

Post-traumatic stress disorder is a concerning psychobehavioral disorder thought to emerge from the complex interaction between genetic and environmental factors. For soldiers exposed to combat, the risk of developing this disorder is twofold and diagnosis is often late, when much sequela has set in. To be able to identify and diagnose in advance those at “risk” of developing post-traumatic stress disorder, would greatly taper the gap between late sequelae and treatment. Therefore, this study sought to determine whether the transcriptome can be used to track the development of post-traumatic stress disorder in this unique and susceptible cohort of individuals. Gene expression levels in peripheral blood samples from 85 Canadian infantry soldiers (n = 58 participants negative for symptoms of post-traumatic stress disorder and n = 27 participants with symptoms of post-traumatic stress disorder) following return from deployment to Afghanistan were determined using RNA sequencing technology. Count-based gene expression quantification, normalization and differential analysis (with thorough correction for confounders) revealed genes associated to PTSD; LRP8 and GOLM1 . These preliminary results provide a proof-of-principle for the diagnostic utility of blood-based gene expression profiles for tracking symptoms of post-traumatic stress disorder in soldiers returning from tour. It is also the first to report transcriptome-wide expression profiles alongside a post-traumatic symptom checklist.

【 授权许可】

CC BY|CC BY-NC